Publisher’s Platform: Will Whole Genome Sequencing Solve More Outbreaks?

“No illnesses have been reported to date.”

How many times have we read a food recall notice posted on either the FDA or FSIS websites and written by the companies recalling the product who use that self-serving statement?  I would say most of the time.

In the past few months the CDC has reported three outbreaks – one Salmonella outbreak and two Listeria outbreaks that have used whole-genome sequencing to connect ill people to tainted product. Perhaps “No illnesses have been reported to date” is a statement of the past trumped by science.

So, what is the science?

State and CDC public health investigators have used the PulseNet system to identify cases of illness that were part of an outbreak for nearly two decades.  PulseNet, the national subtyping network of public health and food regulatory agency laboratories coordinated by CDC, receives from state laboratories DNA “fingerprints” of bacteria obtained through diagnostic testing using Pulsed-field Gel Electrophoresis (PFGE).

Multiple Locus Variable-number Tandem Repeat Analysis (MLVA) is another technique used by scientists to generate a DNA fingerprint for a bacterial isolate. Scientists usually perform MLVA after PFGE to find out more specific details about the type of bacteria that may be causing an outbreak.

Whole Genome Sequencing, is a newer, more highly discriminatory subtyping method, that has been used to define the following outbreaks:

Oasis Brands Inc., Cheese Recalls and Investigation of Human Listeriosis Cases – One person became ill in September 2013 and two persons became ill in June and August 2014. These three ill persons were reported from three states: New York (1), Tennessee (1), and Texas (1).  All ill persons were hospitalized. One death was reported in Tennessee. One illness was related to a pregnancy and was diagnosed in a newborn.

Wholesome Soy Products, Inc. Sprouts Recall and Investigation of Human Listeriosis Cases - Five people became ill from June through August 2014. These five ill people were reported from two states: Illinois (4) and Michigan (1).  All ill people were hospitalized. Two deaths were reported.

Multistate Outbreak of Salmonella Braenderup Infections Linked to Nut Butter Manufactured by nSpired Natural Foods, Inc. - A total of six persons infected with the outbreak strain of Salmonella Braenderup were reported from five states since January 1, 2014.  The number of ill persons identified in each state was as follows: Connecticut (1), Iowa (1), New Mexico (1), Tennessee (1), and Texas (2).

“No illnesses have been reported to date,” may well be a statement of the past.

Food Safety News